Searching for intraspecific chromosomic variation in Passiflora L. species

Comparative cytogenetic studies are very useful in the characterization of different accessions same plant. The aimed this study was search for intraspecific karyotypic variability three species Passiflora genus using chromosomal conventional techniques (Giemsa staining and Fluorochromes base-specific) molecular technique as Fluorescent Situ Hybridization (FISH). diploid character observed all plants analyzed by being 2n = 18 chromosomes number. base-specific heterochromatin only showed GC-rich blocks on satellites nucleolar organizer regions, but no variation found CMA3+/DAPI− blocks, except two P. tholozanii accession, where accession from Manaus five with separate other were found. relation to 45S rDNA located FISH between tholozani accession. nitida coccinea quantitative differentiation sites. Telomeric sites Arabidopsis-type limited terminal suggesting stability. low reinforces need developing new marker mapping increase resolution chromatin visualization.